The bisphosphonates, for their property, are used to inhibit bone resorption. For this reason it is important to support clinical research into bisphosphonates treatment [1] .For clinical purposes it's preferable determinate bisphosphonates in biological samples of human origin with non-radiolabelling methods. This has been a considerable problem in bisphosphonate analysis for several decades. Because nature of bisphosphonates (Table I) there are several analytical difficulties to conduct a non-radiolabelled analysys, i.e. :
1 Bisphosphonates are strongly polar and ionic so it's difficult the extraction with organic solvent;
2 The capacity of complexation with metal ions and other cations of bone matrix which can hamper the analysis;
3 Bisphosphonates are not volatile;
4 The chemical simplicity of especially the older generations of bisphosphonates is not accompanied by any property suitable for sensitive detection in solution.
From:
Rolf W. Sparidans and Jan den Hartigh Chromatographic analysis of bisphosphonates;
Pharm World Sci 1999;21(1): 1-10.
© 1999 Kluwer Academic Publishers. Printed in the Netherlands.
The current procedures for the human biological quantitative determination of bisphosphonates are:
