Inborn errors of metabolism (IEM) research
Quantitation of amino acids and acylcarnitines
Selected reaction monitoring (SRM) method development and verification using triple quadrupole mass spectrometer
Streamlining post-analytical data processing
Nonderivatization vs. derivatization sample preparation method comparison for IEM research
Joseph Di Bussolo, PhD
Evaluating Nonderivatization & Derivatization Tandem MS Methods for Multianalyte Analysis in Dried Blood Spots
Flow injection tandem mass spectrometry (FIA-MS/MS) has been frequently used to analyze amino acids (AA), acylcarnitines (AC), and succinylacetone (SUAC) in dried blood spots (DBS) for inborn errors of metabolism (IEM) research. While butyl esterification method (derivatization) is routine, it is possible to detect AAs, ACs, and SUAC as their native free acids (nonderivatization). In this webinar, we will discuss the research regarding evaluation and comparison of nonderivatization and derivatization sample preparation methods on a triple quadrupole mass spectrometer. The use of meta-calculation software for post-analytical data processing in research will also be discussed.