Autophagosome formation and apoptosome-mediated cell death in neurodevelopment.
The relative roles of the two pathways in neurodevelopment are indicated. Notably, they are both required for neural tube closure
Jessica JE Autophagy
Autophagy in neurodegeneration and development. 2008
Effect of rapamycin on the fate of P23H opsin associated with retinitis pigmentosa (an American Ophthalmological Society thesis).
Trans Am Ophthalmol Soc 2006,Jan,01;104:517-29; (PMID: 17471359) FULL TEXT AVAILABLE
[...]METHODS: Stable cell lines separately expressing WT and P23H opsins and WT and DeltaF508 CFTR were used. Cells were incubated with complete media or amino acid-depleted medium or in the presence of [...]Cells were incubated with complete media or amino acid-depleted medium or in the presence of rapamycin. At various time points thereafter, quantitative opsin and CFTR immunoblotting was performed. Immunofluorescence and electron microscopy were also performed to observe the expression and colocalization of [...]Immunofluorescence and electron microscopy were also performed to observe the expression and colocalization of autophagy specific marker proteins with opsin or CFTR. RESULTS: Upon incubation with rapamycin, the levels of P23H opsin and DeltaF508 CFTR were reduced more rapidly than in untreated controls while no observable changes in the amounts of WT opsin was seen.[...]
Autophagic vacuoles fuse with the prelysosomal compartment in cultured rat fibroblasts.
Eur J Cell Biol 1993,Jun,01;61(1):54-66; (PMID: 8223708)
Punnonen, E L ; Autio, S ; et al.
[...] in lysosomal enzyme delivery to autophagic vacuoles. Firstly, we studied whether endocytic markers, cationized ferritin (CF), bovine serum albumin-gold or horseradish peroxidase (HRP), can be detected in autophagic [...]gold or horseradish peroxidase (HRP), can be detected in autophagic vacuoles. Autophagy was induced by serum removal from the medium with or without leupeptin, an inhibitor of